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A simple and sensitive liquid chromatography-tandem mass spectrometry assay for the quantification of ertapenem in microdialysate.

Abstract : A new liquid chromatography assay with isocratic elution and tandem mass spectrometry detection (LC-MS/MS) using an electrospray ionization interface in the multiple reaction monitoring mode was developed and validated for ertapenem determination in microdialysate samples. Linearity was demonstrated between 10ngmL(-1) (lower limit of quantification, LLoQ) and 160ngmL(-1). The precision (CV%) and accuracy (bias%) in microdialysates at the LLoQ were respectively 2.2% and 17.3% within-day and 10.6% and 2.7% between-days. Ertapenem was stable for 1 month at -20 degrees C and -80 degrees C but unstable at +4 degrees C. This new LC-MS/MS assay is simple, rapid and more sensitive than previously described assays.
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https://www.hal.inserm.fr/inserm-00319493
Contributor : Sandrine Marchand <>
Submitted on : Monday, September 8, 2008 - 3:41:12 PM
Last modification on : Wednesday, January 8, 2020 - 1:38:01 PM
Long-term archiving on: : Saturday, November 26, 2016 - 1:17:43 AM

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Sandrine Lefeuvre, Nicolas Venisse, Sandrine Marchand, Mickael Bachelet, William Couet. A simple and sensitive liquid chromatography-tandem mass spectrometry assay for the quantification of ertapenem in microdialysate.. Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences, Elsevier, 2008, 862 (1-2), pp.242-5. ⟨10.1016/j.jchromb.2007.10.047⟩. ⟨inserm-00319493⟩

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