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Non-invasive in vivo optical imaging of the lacZ and luc gene expression in mice.

Abstract : The bacterial lacZ gene encoding for beta-galactosidase (beta-gal) is a common reporter gene used in transgenic mice. Nonetheless, the absence of fluorigenic substrates usable in live animals greatly hampered the non-invasive follow-up of this reporter gene expression. We used far-red fluorescence for imaging beta-Gal expression in live cells in vitro or in vivo. The 9H-(1,3-dichloro-9,9-dimethylacridin- 2-one-7-yl) beta-D-galactopyranoside substrate was used to monitor beta-Gal expression as a reporter of tumor growth, or of the physiological levels of an endogenous gene or of gene transfer in lung. A quantitative evaluation of this method as well as a comparison of its sensitivity with Firefly Luciferase-based bioluminescence was also performed. In vivo measurements showed that 10(3) beta-Gal tumor cells located under the skin were detectable. In deeper organs like lung, as little as 5 ng of beta-Gal or Luciferase enzymes per mg of proteins were measured, confirming that both techniques reached similar sensibilities. Nonetheless, quantitative comparison of beta-Gal levels measured with far-red imaging or with a standardized enzymatic evaluation after killing revealed that the 2D-fluorescent reflectance imaging method is submitted to a color-dependent disparity of the organs and cannot supply quantitative measurements but that a simple correction can be applied.
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Contributor : Amandine Hurbin Connect in order to contact the contributor
Submitted on : Wednesday, August 27, 2008 - 1:22:55 PM
Last modification on : Friday, November 6, 2020 - 11:18:03 AM

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Véronique Josserand, Isabelle Texier-Nogues, Philippe Huber, Marie C. Favrot, Jean-Luc Coll. Non-invasive in vivo optical imaging of the lacZ and luc gene expression in mice.. Gene Therapy, Nature Publishing Group, 2007, 14 (22), pp.1587-93. ⟨10.1038/⟩. ⟨inserm-00313784⟩



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