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Journal articles
A TFTC/STAGA module mediates histone H2A and H2B deubiquitination, coactivates nuclear receptors, and counteracts heterochromatin silencing.
Yue Zhao
1
Guillaume Lang
2
Saya Ito
1
Jacques Bonnet
2
Eric Metzger
3
Shun Sawatsubashi
1
Eriko Suzuki
1
Xavier Le Guezennec
4
Hendrik Stunnenberg
4
Aleksey Krasnov
5
Sofia Georgieva
5
Roland Schüle
3
Ken-Ichi Takeyama
1
Shigeaki Kato
1
László Tora
2
Didier Devys
2
Hendrik G. Stunnenberg 4
Author
Aleksey Krasnov 5
Author
Sofia G. Georgieva 5
Author
Roland Schüle 3
Author
Ken-Ichi Takeyama 1
Author
Shigeaki Kato 1
Author
László Tora 2
Author IdHAL : laszlo-tora ORCID : https://orcid.org/0000-0001-7398-2250
Didier Devys 2
Author
1
Laboratory of Nuclear Signaling (Japan)
- The University of Tokyo (7 Chome-3-1 Hongo, Bunkyo, Tokyo 113-8654, Japon - Japan)
- Institute of Molecular and Cellular Biosciences (France)
2
IGBMC - Institut de génétique et biologie moléculaire et cellulaire (I.G.B.M.C, Parc D'Innovation 1 Rue Laurent Fries - BP 10142 67404 ILLKIRCH CEDEX - France)
- Université Louis Pasteur - Strasbourg I (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U596 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7104 (France)
3
Universitäts-Frauenklinik und Zentrum für Klinische Forschung (Germany)
- Klinikum der Universität Freiburg (France)
4
Department of Molecular Biology [Nijmegen] (259 RIMLS
Geert Grooteplein 28
6525 GA Nijmegen - Netherlands)
- Radboud University Medical Center [Nijmegen] (P.O. Box 9101 6500 HB Nijmegen - Netherlands)
5
Institute of Gene Biology (Moscow - Russia)
- RAS - Russian Academy of Sciences [Moscow] (Leninsky Ave, 14, Moscow, Russie, 119991 - Russia)
Abstract : Transcriptional activators, several different coactivators, and general transcription factors are necessary to access specific loci in the dense chromatin structure to allow precise initiation of RNA polymerase II (Pol II) transcription. Histone acetyltransferase (HAT) complexes were implicated in loosening the chromatin around promoters and thus in gene activation. Here we demonstrate that the 2 MDa GCN5 HAT-containing metazoan TFTC/STAGA complexes contain a histone H2A and H2B deubiquitinase activity. We have identified three additional subunits of TFTC/STAGA (ATXN7L3, USP22, and ENY2) that form the deubiquitination module. Importantly, we found that this module is an enhancer of position effect variegation in Drosophila. Furthermore, we demonstrate that ATXN7L3, USP22, and ENY2 are required as cofactors for the full transcriptional activity by nuclear receptors. Thus, the deubiquitinase activity of the TFTC/STAGA HAT complex is necessary to counteract heterochromatin silencing and acts as a positive cofactor for activation by nuclear receptors in vivo.
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Journal articles
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https://www.hal.inserm.fr/inserm-00311242
Contributor : Maité Peney <>
Submitted on : Wednesday, August 13, 2008 - 3:38:41 PM
Last modification on : Tuesday, July 14, 2020 - 11:04:04 AM
Contributor : Maité Peney <>
Submitted on : Wednesday, August 13, 2008 - 3:38:41 PM
Last modification on : Tuesday, July 14, 2020 - 11:04:04 AM
Identifiers
- HAL Id : inserm-00311242, version 1
- DOI : 10.1016/j.molcel.2007.12.011
- PUBMED : 18206972
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INSERM | CNRS | IGBMC | SITE-ALSACE
Citation
Yue Zhao, Guillaume Lang, Saya Ito, Jacques Bonnet, Eric Metzger, et al.. A TFTC/STAGA module mediates histone H2A and H2B deubiquitination, coactivates nuclear receptors, and counteracts heterochromatin silencing.. Molecular Cell, Elsevier, 2008, 29 (1), pp.92-101. ⟨10.1016/j.molcel.2007.12.011⟩. ⟨inserm-00311242⟩
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