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Ribosomal position and contacts of mRNA in eukaryotic translation initiation complexes.

Abstract : The position of mRNA on 40S ribosomal subunits in eukaryotic initiation complexes was determined by UV crosslinking using mRNAs containing uniquely positioned 4-thiouridines. Crosslinking of mRNA positions (+)11 to ribosomal protein (rp) rpS2(S5p) and rpS3(S3p), and (+)9-(+)11 and (+)8-(+)9 to h18 and h34 of 18S rRNA, respectively, indicated that mRNA enters the mRNA-binding channel through the same layers of rRNA and proteins as in prokaryotes. Upstream of the P-site, the proximity of positions (-)3/(-)4 to rpS5(S7p) and h23b, (-)6/(-)7 to rpS14(S11p), and (-)8-(-)11 to the 3'-terminus of 18S rRNA (mRNA/rRNA elements forming the bacterial Shine-Dalgarno duplex) also resembles elements of the bacterial mRNA path. In addition to these striking parallels, differences between mRNA paths included the proximity in eukaryotic initiation complexes of positions (+)7/(+)8 to the central region of h28, (+)4/(+)5 to rpS15(S19p), and (-)6 and (-)7/(-)10 to eukaryote-specific rpS26 and rpS28, respectively. Moreover, we previously determined that eukaryotic initiation factor2alpha (eIF2alpha) contacts position (-)3, and now report that eIF3 interacts with positions (-)8-(-)17, forming an extension of the mRNA-binding channel that likely contributes to unique aspects of eukaryotic initiation.
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https://www.hal.inserm.fr/inserm-00311224
Contributor : Maité Peney <>
Submitted on : Wednesday, August 13, 2008 - 3:14:54 PM
Last modification on : Thursday, April 23, 2020 - 2:26:26 PM

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Andrey Pisarev, Victoria Kolupaeva, Marat Yusupov, Christopher Hellen, Tatyana Pestova. Ribosomal position and contacts of mRNA in eukaryotic translation initiation complexes.. EMBO Journal, EMBO Press, 2008, 27 (11), pp.1609-21. ⟨10.1038/emboj.2008.90⟩. ⟨inserm-00311224⟩

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