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Imaging Drosophila pupal wing morphogenesis.

Abstract : Drosophila pupal (P) wing development entails a series of dynamic developmental events, such as epithelial and glial morphogenesis, that are of outstanding interest to cell biologists. Here, we first describe how to prepare P and prepupal (PP) wings for immunofluorescence microscopy. This protocol has been optimized to visualize wing epithelial architecture, such as polarized cortical domains of planar cell polarity proteins. We then provide a protocol to prepare pupae for whole mount live imaging of P wings. This procedure has allowed us to live-image glial cell migration and proliferation along wing sensory nerves.
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Submitted on : Tuesday, August 12, 2008 - 4:56:12 PM
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Anne-Kathrin Classen, Benoît Aigouy, Angela Giangrande, Suzanne Eaton. Imaging Drosophila pupal wing morphogenesis.. Methods in Molecular Biology, Humana Press/Springer Imprint, 2008, 420, pp.265-75. ⟨10.1007/978-1-59745-583-1_16⟩. ⟨inserm-00311091⟩



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