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Cardiac commitment of primate embryonic stem cells

Abstract : Primate nonhuman and human embryonic stem (ES) cells provide a powerful model of early cardiogenesis. Furthermore, engineering of cardiac progenitors or cardiomyocytes from ES cells offers a tool for drug screening in toxicology or to search for molecules to improve and scale up the process of cardiac differentiation using high-throughput screening technology, as well as a source of cell therapy of heart failure. Spontaneous differentiation of ES cells into cardiomyocytes is however limited. Herein, we describe a simple protocol to commit both rhesus and human ES cells toward a cardiac lineage and to sort out early cardiac progenitors. Primate ES cells are challenged for 4 d with the cardiogenic morphogen bone morphogenetic protein 2 (BMP2) and sorted out using anti-SSEA-1 antibody-conjugated magnetic beads. Cardiac progenitor cells can be generated and isolated in 4 d using this protocol.
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Contributor : Michel Puceat Connect in order to contact the contributor
Submitted on : Tuesday, July 15, 2008 - 11:14:36 PM
Last modification on : Wednesday, September 8, 2021 - 1:50:23 PM


  • HAL Id : inserm-00297337, version 1


Julia Leschik, Sonia Stefanovic, Benjamin Brion, Michel Puceat. Cardiac commitment of primate embryonic stem cells. Nature Protocols, Nature Publishing Group, 2008, In press. ⟨inserm-00297337⟩



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