EDEN-dependent translational repression of maternal mRNAs is conserved between Xenopus and Drosophila. - Archive ouverte HAL Access content directly
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 2002

EDEN-dependent translational repression of maternal mRNAs is conserved between Xenopus and Drosophila.

(1) , (2) , (1) , (2) , (2) , (1) , (2)
1
2

Abstract

Translational control is a key level in regulating gene expression in oocytes and eggs because many mRNAs are synthesized and stored during oogenesis for latter use at various stages of oocyte maturation and embryonic development. Understanding the molecular mechanisms that underlie this translational control is therefore crucial. Another important issue is the evolutionary conservation of these mechanisms--in other words the determination of their universal and specific aspects. We report here a comparative analysis of a translational repression mechanism that depends on the EDEN (embryo deadenylation element) element. This small cis-acting element, localized in the 3' untranslated region of c-mos and Eg mRNAs, was shown to be involved in a deadenylation process. We demonstrate here that in Xenopus embryos, mRNAs that contain an EDEN are translationally repressed. Next, transgenic flies were used to study the effect of the EDEN motif on translation in Drosophila oocytes. We show that this element also causes the translational repression of a reporter gene in Drosophila demonstrating that the EDEN-dependent translational repression is functionally conserved between Xenopus and Drosophila.
Embargoed file
Embargoed file
Ne sera jamais visible

Dates and versions

inserm-00292951 , version 1 (03-07-2008)

Identifiers

Cite

Nader Ezzeddine, Luc Paillard, Michèle Capri, Dominique Maniey, Thérèse Bassez, et al.. EDEN-dependent translational repression of maternal mRNAs is conserved between Xenopus and Drosophila.. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99 (1), pp.257-62. ⟨10.1073/pnas.012555499⟩. ⟨inserm-00292951⟩
109 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More