Skip to Main content Skip to Navigation
Journal articles

EDEN-dependent translational repression of maternal mRNAs is conserved between Xenopus and Drosophila.

Abstract : Translational control is a key level in regulating gene expression in oocytes and eggs because many mRNAs are synthesized and stored during oogenesis for latter use at various stages of oocyte maturation and embryonic development. Understanding the molecular mechanisms that underlie this translational control is therefore crucial. Another important issue is the evolutionary conservation of these mechanisms--in other words the determination of their universal and specific aspects. We report here a comparative analysis of a translational repression mechanism that depends on the EDEN (embryo deadenylation element) element. This small cis-acting element, localized in the 3' untranslated region of c-mos and Eg mRNAs, was shown to be involved in a deadenylation process. We demonstrate here that in Xenopus embryos, mRNAs that contain an EDEN are translationally repressed. Next, transgenic flies were used to study the effect of the EDEN motif on translation in Drosophila oocytes. We show that this element also causes the translational repression of a reporter gene in Drosophila demonstrating that the EDEN-dependent translational repression is functionally conserved between Xenopus and Drosophila.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00292951
Contributor : Howard Beverley Osborne <>
Submitted on : Thursday, July 3, 2008 - 10:59:06 AM
Last modification on : Wednesday, July 1, 2020 - 2:50:03 PM
Long-term archiving on: : Saturday, November 26, 2016 - 12:27:10 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Citation

Nader Ezzeddine, Luc Paillard, Michèle Capri, Dominique Maniey, Thérèse Bassez, et al.. EDEN-dependent translational repression of maternal mRNAs is conserved between Xenopus and Drosophila.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2002, 99 (1), pp.257-62. ⟨10.1073/pnas.012555499⟩. ⟨inserm-00292951⟩

Share

Metrics

Record views

262