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An analysis of the sequence requirements of EDEN-BP for specific RNA binding.

Abstract : EDEN-BP (embryo deadenylation element-binding protein) binds specifically to the EDEN motif in the 3'-untranslated regions of maternal mRNAs and targets these mRNAs for deadenylation and translational repression in Xenopus laevis embryos. EDEN-BP contains three RNA recognition motifs (RRMs) and is related to the elav family of RNA-binding proteins. In the present study we show that the two N-terminal RRMs of EDEN-BP are necessary for the interaction with EDEN as well as a part of the linker region (between RRM2 and RRM3). Using a band shift assay we show that two different complexes are formed according to the size and, therefore, the functional nature of the EDEN motif. Finally, we show that EDEN-BP can form a dimer in a two-hybrid assay. Accordingly, we suggest that the functional configuration of EDEN-BP is a dimer.
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Submitted on : Thursday, July 3, 2008 - 10:24:21 AM
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Sylvie Bonnet-Corven, Yann Audic, Francis Omilli, Howard Beverley Osborne. An analysis of the sequence requirements of EDEN-BP for specific RNA binding.. Nucleic Acids Research, Oxford University Press, 2002, 30 (21), pp.4667-74. ⟨10.1093/nar/gkf586⟩. ⟨inserm-00292945⟩



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