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Journal articles
An analysis of the sequence requirements of EDEN-BP for specific RNA binding.
Abstract : EDEN-BP (embryo deadenylation element-binding protein) binds specifically to the EDEN motif in the 3'-untranslated regions of maternal mRNAs and targets these mRNAs for deadenylation and translational repression in Xenopus laevis embryos. EDEN-BP contains three RNA recognition motifs (RRMs) and is related to the elav family of RNA-binding proteins. In the present study we show that the two N-terminal RRMs of EDEN-BP are necessary for the interaction with EDEN as well as a part of the linker region (between RRM2 and RRM3). Using a band shift assay we show that two different complexes are formed according to the size and, therefore, the functional nature of the EDEN motif. Finally, we show that EDEN-BP can form a dimer in a two-hybrid assay. Accordingly, we suggest that the functional configuration of EDEN-BP is a dimer.
Cited literature [46 references]
https://www.hal.inserm.fr/inserm-00292945
Contributor : Howard Beverley Osborne <>
Submitted on : Thursday, July 3, 2008 - 10:24:21 AM
Last modification on : Thursday, January 14, 2021 - 11:17:13 AM
Long-term archiving on: : Friday, May 28, 2010 - 11:07:44 PM
Contributor : Howard Beverley Osborne <>
Submitted on : Thursday, July 3, 2008 - 10:24:21 AM
Last modification on : Thursday, January 14, 2021 - 11:17:13 AM
Long-term archiving on: : Friday, May 28, 2010 - 11:07:44 PM
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NAR_2002.pdf
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