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Article Dans Une Revue Circulation Research Année : 2008

Bone morphogenetic protein-9 is a circulating vascular quiescence factor.

Résumé

Angiogenesis is a complex process, requiring a finely tuned balance between numerous stimulatory and inhibitory signals. ALK1 (activin receptor like-kinase 1) is an endothelial-specific type 1 receptor of the transforming growth factor-beta receptor family. Heterozygotes with mutations in the ALK1 gene develop hereditary hemorrhagic telangiectasia type 2 (HHT2). Recently, we reported that bone morphogenetic protein (BMP)9 and BMP10 are specific ligands for ALK1 that potently inhibit microvascular endothelial cell migration and growth. These data lead us to suggest that these factors may play a role in the control of vascular quiescence. To test this hypothesis, we checked their presence in human serum. We found that human serum induced Smad1/5 phosphorylation. To identify the active factor, we tested neutralizing antibodies against BMP members and found that only the anti-BMP9 inhibited serum-induced Smad1/5 phosphorylation. The concentration of circulating BMP9 was found to vary between 2 and 12 ng/mL in sera and plasma from healthy humans, a value well above its EC(50) (50 pg/mL). These data indicated that BMP9 is circulating at a biologically active concentration. We then tested the effects of BMP9 in 2 in vivo angiogenic assays. We found that BMP9 strongly inhibited sprouting angiogenesis in the mouse sponge angiogenesis assay and that BMP9 could inhibit blood circulation in the chicken chorioallantoic membrane assay. Taken together, our results demonstrate that BMP9, circulating under a biologically active form, is a potent antiangiogenic factor that is likely to play a physiological role in the control of adult blood vessel quiescence.

Mots clés

BMP9 ALK1 HHT angiogenesis

Domaines

Biologie cellulaire
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https://inserm.hal.science/inserm-00277236

Soumis le : vendredi 29 août 2008-09:01:47

Dernière modification le : vendredi 19 avril 2024-16:18:56

Archivage à long terme le : vendredi 28 mai 2010-17:16:42

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inserm-00277236 , version 1 (29-08-2008)

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Citer

Laurent David, Christine Mallet, Michelle Keramidas, Noël Lamandé, Jean-Marie Gasc, et al.. Bone morphogenetic protein-9 is a circulating vascular quiescence factor.: BMP9 is present in serum and inhibits angiogenesis. Circulation Research, 2008, 102 (8), pp.914-22. ⟨10.1161/CIRCRESAHA.107.165530⟩. ⟨inserm-00277236⟩

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