Evaluation of endothelial cell adhesion onto different protein/gold electrodes by EIS. - Archive ouverte HAL Access content directly
Journal Articles Macromolecular Bioscience Year : 2007

Evaluation of endothelial cell adhesion onto different protein/gold electrodes by EIS.

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Abstract

To study cell attachment to biomaterials, several proteins such as fibronectin, collagen IV, heparin, immunoglobulin G, and albumin have been deposited onto polystyrene adsorbed on a self-assembled monolayer (silane or thiol) on glass or gold, respectively. The different steps of this multilayer assembly have been characterized by electrochemical impedance spectroscopy (EIS). These data are compared to those of adhesion rate, viability percentage, and cytoskeleton labeling for a better understanding of the cell adhesion process to each protein. All the proteins are endothelial cell adhering biomolecules but not with the same features. A linear relationship has been established between adhesion rate and resistance of the endothelial cell/protein interface for all negatively charged proteins.
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inserm-00276758 , version 1 (02-05-2008)

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Amira Bouafsoun, Saloua Helali, Ali Othmane, Abdelhamid Kerkeni, Anne-France Prigent, et al.. Evaluation of endothelial cell adhesion onto different protein/gold electrodes by EIS.. Macromolecular Bioscience, 2007, 7 (5), pp.599-610. ⟨10.1002/mabi.200600263⟩. ⟨inserm-00276758⟩
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