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V1a vasopressin receptor expression is modulated during myogenic differentiation.

Abstract : Neurohypophyseal peptides potently stimulate myogenic differentiation by acting through different receptors of the same family. Here, we show that L6C5 myogenic cells express, at a high density, a single class of V1a Arg8-vasopressin (AVP) receptor. The expression of the vasopressin receptor of type 1a (V1aR) is significantly higher in proliferating myoblasts than in differentiated myotubes. The differentiation-related decrease of V1aR expression was evident both at the mRNA and at the protein level as shown by the reduction of [(3)H]-AVP binding. However, in L6C5 cells transfected with a synthetic construct containing the luciferase gene driven by the 2 kb upstream region of V1aR, we observed a stimulation of the activity of the promoter when the cells were cultured in differentiative medium. The down-regulation of the V1aR correlated with a decreased half-life of its mRNA (half-life 5.86+/-0.74 hr in 10% fetal bovine serum [FBS] versus 3.53+/-0.72 hr in 1% FBS). Cyclosporine A and dexamethasone, but not 5'-azacytidine, treatments of cells in differentiation medium restored the V1aR level to that measured in proliferating L6C5 cells, thus confirming the role of post-transcriptional mechanisms in the modulation of V1aR expression. Taken together, these data show that mRNA stability plays a role in modulating protein expression during the myogenic differentiation process.
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Contributor : Evelyne Vericel <>
Submitted on : Friday, May 2, 2008 - 8:26:02 AM
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Monica Alvisi, Valeria de Arcangelis, Letizia Ciccone, Valeria Palombi, Marta Alessandrini, et al.. V1a vasopressin receptor expression is modulated during myogenic differentiation.. Differentiation / The Journal of the International Society of Differentiation, 2008, 76 (4), pp.371-80. ⟨10.1111/j.1432-0436.2007.00231.x⟩. ⟨inserm-00276755⟩



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