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A red wine polyphenolic extract reduces the activation phenotype of cultured human liver myofibroblasts.

Abstract : AIM: To test the effect of a standardized red wine polyphenolic extract (RWPE) on the phenotype of human liver myofibroblasts in culture. METHODS: Human myofibroblasts grown from liver explants were used in this study. Cell proliferation was measured with the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. Signaling events were analyzed by western blot with phospho-specific antibodies. Matrix-metalloproteinase activity was measured with gel zymography. RESULTS: We found that cell proliferation was dose-dependently decreased by up to 90% by RWPE while cell viability was not affected. Exposure to RWPE also greatly decreased the phosphorylation of ERK1/ERK2 and Akt in response to stimulation by the mitogenic factor platelet-derived growth factor BB (PDGF-BB). Finally, RWPE affected extracellular matrix remodeling by decreasing the secretion by myofibroblasts of matrix-metalloproteinase-2 and of tissue inhibitor of matrix-metalloproteinases-1. CONCLUSION: Altogether, RWPE decreases the activation state of liver myofibroblasts. The identification of the active compounds in RWPE could offer new therapeutic strategies against liver fibrosis.
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https://www.hal.inserm.fr/inserm-00275294
Contributor : Jean Rosenbaum <>
Submitted on : Tuesday, November 10, 2009 - 3:37:19 PM
Last modification on : Wednesday, November 29, 2017 - 2:54:20 PM
Long-term archiving on: : Friday, May 28, 2010 - 5:17:31 PM

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  • HAL Id : inserm-00275294, version 1
  • PUBMED : 18407593

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Véronique Neaud, Jean Rosenbaum. A red wine polyphenolic extract reduces the activation phenotype of cultured human liver myofibroblasts.. World Journal of Gastroenterology, Baishideng Publishing Group Co. Limited, 2008, 14 (14), pp.2194-9. ⟨inserm-00275294⟩

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