MHC Class II tetramers and the pursuit of antigen-specific T cells: define, deviate, delete. - Archive ouverte HAL Access content directly
Journal Articles Clinical Immunology Year : 2004

MHC Class II tetramers and the pursuit of antigen-specific T cells: define, deviate, delete.

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Abstract

Selective expansion and activation of a very small number of antigen-specific CD4(+) T cells is a remarkable and essential property of the adaptive immune response. Antigen-specific T cells were until recently identified only indirectly by functional assays, such as antigen-induced cytokine secretion and proliferation. The advent of MHC Class II tetramers has added a pivotal tool to our research armamentarium, allowing the definition of allo- and autoimmune responses in deeper detail. Rare antigen-specific CD4(+) cells can now be selectively identified, isolated and characterized. The same tetramer reagents also provide a new mean of stimulating T cells, more closely reproducing the MHC-peptide/TCR interaction. This property allows the use of tetramers to direct T cells toward the more desirable outcome, that is, activation (in malignancies and infectious diseases) or Th2/T regulatory cell deviation, anergy and deletion (in autoimmune diseases). These experimental approaches hold promise for diagnostic, prognostic and therapeutic applications.
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Dates and versions

inserm-00266546 , version 1 (26-03-2008)

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Roberto Mallone, Gerald T Nepom. MHC Class II tetramers and the pursuit of antigen-specific T cells: define, deviate, delete.: MHC Class II tetramers. Clinical Immunology, 2004, 110 (3), pp.232-42. ⟨10.1016/j.clim.2003.11.004⟩. ⟨inserm-00266546⟩

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