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iNOS is a mediator of the heat stress-induced preconditioning against myocardial infarction in vivo in the rat.

Abstract : OBJECTIVE: The inducible isoform of nitric oxide synthase (iNOS) is known to be a trigger of the heat stress (HS)-induced cardioprotection. Since iNOS also appears to mediate various forms of myocardial preconditioning, the goal of this study was to investigate its role as a mediator of the HS response. METHODS AND RESULTS: Male Wistar rats were divided in six groups, subjected or not to HS (42 degrees C internal temperature, for 15 min). Twenty-four hours later, they were treated or not with either L-NAME, a non-selective inhibitor of NO synthase isoforms, or 1400W, a selective iNOS inhibitor, 10 min before being subjected to a 30-min left coronary artery occlusion followed by a 120-min reperfusion, in vivo. The infarct size (tetrazolium staining) reducing effect conferred by heat stress (from 46.0+/-1.4% in sham to 26.8+/-3.8% in HS groups) was completely abolished by both L-NAME (53.9+/-3.1%) and 1400W (51.8+/-3.3%). Additional studies using Western blot analysis demonstrated a 3.8-fold increase in myocardial iNOS protein expression 24 h after HS. CONCLUSION: These results suggest an involvement of iNOS as a mediator of the protection conferred by heat stress against myocardial ischaemia.
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https://www.hal.inserm.fr/inserm-00266323
Contributor : Claire Arnaud <>
Submitted on : Friday, March 21, 2008 - 4:16:47 PM
Last modification on : Friday, November 6, 2020 - 4:04:46 AM
Long-term archiving on: : Thursday, May 20, 2010 - 10:22:52 PM

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  • HAL Id : inserm-00266323, version 1
  • PUBMED : 12667952

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Claire Arnaud, Diane Godin-Ribuot, Serge Bottari, André Peinnequin, Marie Joyeux, et al.. iNOS is a mediator of the heat stress-induced preconditioning against myocardial infarction in vivo in the rat.. Cardiovascular Research, Oxford University Press (OUP), 2003, 58 (1), pp.118-25. ⟨inserm-00266323⟩

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