Cell adaptive response to extracellular matrix density is controlled by ICAP-1-dependent beta1-integrin affinity. - Archive ouverte HAL Access content directly
Journal Articles Journal of Cell Biology Year : 2008

Cell adaptive response to extracellular matrix density is controlled by ICAP-1-dependent beta1-integrin affinity.

(1, 2) , (1, 2) , (1) , (1, 2) , (2) , (2)
1
2

Abstract

Cell migration is an integrated process requiring the continuous coordinated assembly and disassembly of adhesion structures. How cells orchestrate adhesion turnover is only partially understood. We provide evidence for a novel mechanistic insight into focal adhesion (FA) dynamics by demonstrating that integrin cytoplasmic domain-associated protein 1 (ICAP-1) slows down FA assembly. Live cell imaging, which was performed in both Icap-1-deficient mouse embryonic fibroblasts and cells expressing active beta(1) integrin, shows that the integrin high affinity state favored by talin is antagonistically controlled by ICAP-1. This affinity switch results in modulation in the speed of FA assembly and, consequently, of cell spreading and migration. Unexpectedly, the ICAP-1-dependent decrease in integrin affinity allows cell sensing of matrix surface density, suggesting that integrin conformational changes are important in mechanotransduction. Our results clarify the function of ICAP-1 in cell adhesion and highlight the central role it plays in the cell's integrated response to the extracellular microenvironment.
Fichier principal
Vignette du fichier
JCB_200707142_1_s_2.pdf (2.24 Mo) Télécharger le fichier
Loading...

Dates and versions

inserm-00263537 , version 1 (12-03-2008)

Identifiers

Cite

Angélique Millon-Frémillon, Daniel Bouvard, Alexei Grichine, Sandra Manet-Dupé, Marc R. Block, et al.. Cell adaptive response to extracellular matrix density is controlled by ICAP-1-dependent beta1-integrin affinity.. Journal of Cell Biology, 2008, 180 (2), pp.427-41. ⟨10.1083/jcb.200707142⟩. ⟨inserm-00263537⟩
91 View
166 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More