Effect of rebamipide on the colonic barrier in interleukin 10 deficient mice
Résumé
Our aim was to study the effect of a mucosal protective agent, rebamipide, on the colonic barrier and the immune response in colitis-prone interleukin-10 deficient (IL-10-/-) C57BL/6 mice infected with Helicobacter hepaticus. After sacrifice, in all mice, control, or previously infected with H. hepaticus, or previously infected and treated with rebamipide enema, a histological examination of colonic samples was performed, intestinal permeability was studied in Ussing chamber, and mesenteric lymph node proliferation and cytokine secretion were measured. Mice treated with rebamipide, presented a reinforcement of the distal colonic epithelial barrier, an increase of mesenteric lymph node cells proliferation and of IFNΓ and IL-12 secretion. These results indicate that in IL-10-/- mice with mild colitis, rectally administered rebamipide reinforces the distal colonic barrier and has a slight Th1 immuno-stimulatory effect on mesenteric lymph node cells. These properties could be helpful in the management of some inflammatory bowel diseases.
Domaines
Immunologie
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Art_Reb_Dig_Dis_Sci_figures.pdf (60.03 Ko)
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Article_Rebamipide_Dig_Dis_Sci_Revised-Final_3_Nov_05.pdf (335.81 Ko)
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