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Uptake of host cell transforming growth factor-ß by Trypanosoma cruzi amastigotes in cardiomyocytes: potential role in parasite cycle completion.: Cycle-dependent uptake of TGF-ß by T. cruzi

Abstract : Transforming growth factor-β (TGF-β) is a cytokine that plays various functions in the control of Trypanosoma cruzi infectivity and in the progression of Chagas disease. When we immunostained Trypanosoma cruzi-infected cardiomyocytes (following either in vivo or in vitro infections) for TGF-β, we observed stronger immunoreactivity in parasites than in host cells. TGF-β immunoreactivity evolved during parasite cycle progression: intense staining in amastigotes versus very faint staining in trypomastigotes. TGF-β was present on the surface of amastigotes, in the flagellar pocket and in intraparasitic vesicles as revealed by electron microscopy. However, no ortholog TGF-β gene could be identified in the genome of Trypanosoma cruzi by in silico analysis or by extensive PCR and RT-PCR studies. Immunoreactive TGF-β was most probably taken up by the parasite from the host cell cytoplasm since such an internalization process of biotinylated TGF-β could be observed in axenic amastigotes in vitro. These observations represent the first example of a novel mechanism by which a primitive unicellular protozoan can use host cell TGF-β to control its own intracellular cycle.
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https://www.hal.inserm.fr/inserm-00207611
Contributor : Jean-Jacques Feige <>
Submitted on : Thursday, January 17, 2008 - 6:23:45 PM
Last modification on : Tuesday, March 17, 2020 - 3:34:39 AM
Long-term archiving on: : Tuesday, April 13, 2010 - 7:01:11 PM

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Mariana Waghabi, Michelle Keramidas, Sabine Bailly, Wim Degrave, Leila Mendonça-Lima, et al.. Uptake of host cell transforming growth factor-ß by Trypanosoma cruzi amastigotes in cardiomyocytes: potential role in parasite cycle completion.: Cycle-dependent uptake of TGF-ß by T. cruzi. American Journal of Pathology, American Society for Investigative Pathology, 2005, 167 (4), pp.993-1003. ⟨10.1016/s0002-9440(10)61189-3⟩. ⟨inserm-00207611⟩

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