Skip to Main content Skip to Navigation
Journal articles

Loss of Sugar Detection by GLUT2 Affects Glucose Homeostasis in Mice.

Abstract : BACKGROUND: Mammals must sense the amount of sugar available to them and respond appropriately. For many years attention has focused on intracellular glucose sensing derived from glucose metabolism. Here, we studied the detection of extracellular glucose concentrations in vivo by invalidating the transduction pathway downstream from the transporter-detector GLUT2 and measured the physiological impact of this pathway. METHODOLOGY/PRINCIPAL FINDINGS: We produced mice that ubiquitously express the largest cytoplasmic loop of GLUT2, blocking glucose-mediated gene expression in vitro without affecting glucose metabolism. Impairment of GLUT2-mediated sugar detection transiently protected transgenic mice against starvation and streptozotocin-induced diabetes, suggesting that both low- and high-glucose concentrations were not detected. Transgenic mice favored lipid oxidation, and oral glucose was slowly cleared from blood due to low insulin production, despite massive urinary glucose excretion. Kidney adaptation was characterized by a lower rate of glucose reabsorption, whereas pancreatic adaptation was associated with a larger number of small islets. CONCLUSIONS/SIGNIFICANCE: Molecular invalidation of sugar sensing in GLUT2-loop transgenic mice changed multiple aspects of glucose homeostasis, highlighting by a top-down approach, the role of membrane glucose receptors as potential therapeutic targets.
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00199358
Contributor : Maude Le Gall <>
Submitted on : Tuesday, December 18, 2007 - 6:00:56 PM
Last modification on : Saturday, May 30, 2020 - 2:12:49 PM
Long-term archiving on: : Monday, April 12, 2010 - 8:23:50 AM

Identifiers

Citation

Emilie Stolarczyk, Maude Le Gall, Patrick Even, Anne Houllier, Patricia Serradas, et al.. Loss of Sugar Detection by GLUT2 Affects Glucose Homeostasis in Mice.. PLoS ONE, Public Library of Science, 2007, 2 (12), pp.e1288. ⟨10.1371/journal.pone.0001288⟩. ⟨inserm-00199358⟩

Share

Metrics

Record views

592

Files downloads

1244