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Dp71, utrophin and beta-dystroglycan expression and distribution in PC12/L6 cell cocultures.

Abstract : Function of dystrophin Dp71 isoforms is unknown but seems related to neurite outgrowth and synapse formation. To evaluate Dp71 role in myoneural synapses, we established a coculture model using PC12 cells and L6 myotubes and analyzed expression and localization of Dp71 and related proteins, utrophin and beta-dystroglycan, in PC12 cells. Confocal microscopy showed Dp71d isoform in PC12 nuclei, golgi-complex-like and endoplasmic reticulum-like structures, whereas Dp71ab concentrates at neurite tips and cytoplasm, colocalizing with beta-dystroglycan, utrophin, synaptophysin and acetylcholine receptors. Evidences suggest that Dp71ab isoform, unlike Dp71d, may take part in neurite-related processes. This is the first work on Dp and members of Dp-associated protein complex roles in a cell-line based coculturing system, which may be useful in determining Dp71 isoforms associations.
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Contributor : Dominique Mornet Connect in order to contact the contributor
Submitted on : Tuesday, October 28, 2008 - 10:40:29 AM
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Ramses Ilarraza-Lomeli, Bulmaro Cisneros-Vega, Maria de Lourdes Cervantes-Gomez, Dominique Mornet, Cecilia Montañez. Dp71, utrophin and beta-dystroglycan expression and distribution in PC12/L6 cell cocultures.. NeuroReport, Lippincott, Williams & Wilkins, 2007, 18 (16), pp.1657-61. ⟨10.1097/WNR.0b013e3282f0e42d⟩. ⟨inserm-00191063⟩



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