Identification of the L,D-transpeptidases responsible for attachment of the Braun lipoprotein to Escherichia coli peptidoglycan.

Sophie Magnet; Samuel Bellais; Lionel Dubost; Martine Fourgeaud; Jean-Luc Mainardi; Sébastien Petit-Frère; Arul Marie; Dominique Mengin-Lecreulx; Michel Arthur; Laurent Gutmann

doi:10.1128/JB.00084-07

Journal articles

Identification of the L,D-transpeptidases responsible for attachment of the Braun lipoprotein to Escherichia coli peptidoglycan.

Sophie Magnet ^{1, *} Samuel Bellais ¹ Lionel Dubost ² Martine Fourgeaud ¹ Jean-Luc Mainardi ^{1, 3} Sébastien Petit-Frère ¹ Arul Marie ² Dominique Mengin-Lecreulx ⁴ Michel Arthur ¹ Laurent Gutmann ^{1, 3}

* Corresponding author

1 Laboratoire de Recherche Moléculaire sur les Antibiotiques

2 Laboratoire de chimie et biochimie des substances naturelles

3 HEGP - Hôpital Européen Georges Pompidou [APHP]

4 ENVBAC - Enveloppes Bactériennes et Antibiotiques

Dpt Microbio - Département Microbiologie

Abstract : The L,D-transpeptidase Ldt(fm) catalyzes peptidoglycan cross-linking in beta-lactam-resistant mutant strains of Enterococcus faecium. Here, we show that in Escherichia coli Ldt(fm) homologues are responsible for the attachment of the Braun lipoprotein to murein, indicating that evolutionarily related domains have been tailored to use muropeptides or proteins as acyl acceptors in the L,D-transpeptidation reaction.

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Biochemistry, Molecular Biology

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https://www.hal.inserm.fr/inserm-00185689
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Submitted on : Tuesday, November 6, 2007 - 4:58:16 PM
Last modification on : Friday, September 11, 2020 - 3:01:05 AM
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Identification of the L,D-transpeptidases responsible for attachment of the Braun lipoprotein to Escherichia coli peptidoglycan.

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Identification of the L,D-transpeptidases responsible for attachment of the Braun lipoprotein to Escherichia coli peptidoglycan.

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