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UVB-induced G2 arrest of human melanocytes involves Cdc2 sequestration by Gadd45a in nuclear speckles.

Abstract : Exposure to solar UVB radiation is involved in the development of cutaneous melanoma. We previously showed that human melanocytes and melanoma cells respond to UVB radiation via a p53-independent pathway involving GADD45A activation. Here, we determined that UVB-induction of Gadd45a is necessary for G(2) arrest and that Gadd45a and its partner p21(Waf1) colocalize in nuclear bodies called Nuclear Speckles. We further observed that UVB-induced G(2) arrest is associated with Cdc2 accumulation in these Nuclear Speckles. Knock-down of Gadd45a expression by RNA interference prevents both UVB-induced Cdc2 accumulation in Nuclear Speckles and G(2) arrest. Our results demonstrate that UVB-induced G(2) arrest of melanoma cells is Gadd45a-dependent. Furthermore, we show that Cdc2 sequestration by Gadd45a occurs in Nuclear Speckles, suggesting a new role for these nuclear bodies, so far only linked to RNA maturation.
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https://www.hal.inserm.fr/inserm-00175854
Contributor : Danielle Salas <>
Submitted on : Monday, October 1, 2007 - 3:14:43 PM
Last modification on : Thursday, November 21, 2019 - 2:26:20 AM

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  • HAL Id : inserm-00175854, version 1
  • PUBMED : 16931908

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Caroline Fayolle, Julie Pourchet, Aurélie Cohen, Rémy Pedeux, Alain Puisieux, et al.. UVB-induced G2 arrest of human melanocytes involves Cdc2 sequestration by Gadd45a in nuclear speckles.. Cell Cycle, Taylor & Francis, 2006, 5 (16), pp.1859-64. ⟨inserm-00175854⟩

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