Skip to Main content Skip to Navigation
Journal articles

A role for PKCzeta in the LPS-induced translocation NF-kappaB p65 subunit in cultured myometrial cells.

Abstract : Human myometrial cells respond to the endotoxin lipopolysaccharide (LPS) by activation of protein kinase C (PKC) zeta and nuclear translocation of the p65 subunit of NF-kB. Our first objective was to determine the expression of TLR4 in cultured myometrial cells. Positive immunoreactivity observed for TLR4 suggests that myometrial cells have the potential to respond to LPS. To confirm that LPS signals via TLR4, the ability of an anti-TLR4 neutralizing antibody to block LPS-induced translocation of p65 was demonstrated. To determine whether LPS-induced nuclear translocation of p65 is mediated through the PKC pathway, myometrial cells were treated with various inhibitors of the PKC isoforms already characterized in human myometrium. Neither the selective conventional PKC inhibitor nor the inhibitor of PKCdelta affected NF-kB activation. By contrast, we found that treatment of myometrial cells with an antisense against PKCzeta affect LPS-induced nuclear translocation of the p65 subunit of NF-kB. Accordingly, our data support the notion that PKCzeta is essential for LPS-induced NF-kB p65 subunit nuclear translocation in human myometrial cells.
Document type :
Journal articles
Complete list of metadatas

Cited literature [48 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00171770
Contributor : Michelle Breuiller-Fouche <>
Submitted on : Thursday, September 13, 2007 - 10:41:42 AM
Last modification on : Tuesday, September 22, 2020 - 3:58:59 AM
Long-term archiving on: : Friday, November 25, 2016 - 7:01:29 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Emmanuelle Dallot, Céline Méhats, Stéphanie Oger, Marie-Josèphe Leroy, Michelle Breuiller-Fouché. A role for PKCzeta in the LPS-induced translocation NF-kappaB p65 subunit in cultured myometrial cells.. Biochimie, Elsevier, 2005, 87 (6), pp.513-21. ⟨10.1016/j.biochi.2005.02.009⟩. ⟨inserm-00171770⟩

Share

Metrics

Record views

377