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Comprehensive profiling of 8p11-12 amplification in breast cancer.

Abstract : In human carcinomas, especially breast cancer, chromosome arm 8p is frequently involved in complex chromosomal rearrangements that combine amplification at 8p11-12, break in the 8p12-21 region, and loss of 8p21-ter. Several studies have identified putative oncogenes in the 8p11-12 amplicon. However, discrepancies and the lack of knowledge on the structure of this amplification lead us to think that the actual identity of the oncogenes is not definitively established. We present here a comprehensive study combining genomic, expression, and chromosome break analyses of the 8p11-12 region in breast cell lines and primary breast tumors. We show the existence of four amplicons at 8p11-12 using array comparative genomic hybridization. Gene expression analysis of 123 samples using DNA microarrays identified 14 genes significantly overexpressed in relation to amplification. Using fluorescence in situ hybridization analysis on tissue microarrays, we show the existence of a cluster of breakpoints spanning a region just telomeric to and associated with the amplification. Finally, we show that 8p11-12 amplification has a pejorative effect on survival in breast cancer.
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https://www.hal.inserm.fr/inserm-00158812
Contributor : Yves Le Ster <>
Submitted on : Friday, June 29, 2007 - 4:25:05 PM
Last modification on : Friday, December 21, 2018 - 1:19:57 AM
Long-term archiving on: : Friday, November 25, 2016 - 3:20:13 PM

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Véronique Gelsi-Boyer, Béatrice Orsetti, Nathalie Cervera, Pascal Finetti, Fabrice Sircoulomb, et al.. Comprehensive profiling of 8p11-12 amplification in breast cancer.. Molecular Cancer Research, American Association for Cancer Research, 2005, 3 (12), pp.655-67. ⟨10.1158/1541-7786.MCR-05-0128⟩. ⟨inserm-00158812⟩

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