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X-ray microanalysis of airway surface liquid collected in cystic fibrosis mice.

Abstract : The airway surface liquid (ASL) that lines the airway surface epithelium plays a major role in airway antibacterial defense and mucociliary transport efficiency, two key factors in cystic fibrosis (CF) disease. A major difficulty is to collect ASL in native conditions without stimulation or alteration of the underlying airway epithelium. Using a cryoprobe specifically adapted to collect native ASL from the tracheal mouse surface, we analyzed by X-ray microanalysis the complete ASL and plasma ion content in Cftr(tm1Hgu)/Cftr(tm1Hgu) mice compared with that in control littermates. ASL ion content from eight Cftr(tm1Hgu)/Cftr(tm1Hgu) mice and eight control littermates did not appear significantly different. The mean (+/-SE) concentrations were 2,352 +/- 367 and 2,058 +/- 401 mmol/kg dry weight for Na, 1,659 +/- 272 and 1,448 +/- 281 mmol/kg dry weight for Cl, 357 +/- 57 and 337 +/- 38 mmol/kg dry weight for S, 1,066 +/- 220 and 787 +/- 182 mmol/kg dry weight for K, 400 +/- 82 and 301 +/- 58 mmol/kg dry weight for Ca, 105 +/- 31 and 105 +/- 20 mmol/kg dry weight for Mg, 33 +/- 15 and 29 +/- 9 mmol/kg dry weight for P in non-CF and CF mice, respectively. This cryotechnique appears to be a promising technique for analyzing the complete elemental composition of native ASL in CF and non-CF tissues.
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Contributor : Philippe Birembaut <>
Submitted on : Thursday, June 7, 2007 - 10:02:34 AM
Last modification on : Wednesday, August 30, 2017 - 1:13:11 AM


  • HAL Id : inserm-00152532, version 1
  • PUBMED : 11435203



Jm Zahm, S. Baconnais, D. Davidson, S. Webb, J. Dorin, et al.. X-ray microanalysis of airway surface liquid collected in cystic fibrosis mice.. American Journal of Physiology - Lung Cellular and Molecular Physiology, American Physiological Society, 2001, 281 (2), pp.L309-13. ⟨inserm-00152532⟩



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