Improved activity of an actin-resistant DNase I variant on the cystic fibrosis airway secretions. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue American Journal of Respiratory and Critical Care Medicine Année : 2001

Improved activity of an actin-resistant DNase I variant on the cystic fibrosis airway secretions.

Résumé

In cystic fibrosis (CF), actin and DNA originating from inflammatory cells contribute to the thickness of airway secretions. Actin can bind to DNA-rich fibers and potently inhibit the enzymatic activity of rhDNase. The in vitro effects of the actin-resistant rhDNase variant (A114R) were analyzed and compared with those of the wild-type rhDNase. Frozen and thawed CF airway secretions were incubated for 30 min with different concentrations (0.1, 0.5, 1, 5, or 10 microg/ml) of either actin-resistant rhDNase or wild-type rhDNase. We observed that both the wild-type and the actin-resistant rhDNase significantly decreased (p < 0.05 and p < 0.001, respectively) the airway secretion viscosity. The decrease in airway secretion viscosity was significant even at low concentrations (0.1 microg/ml) of the actin-resistant variant. Incubation with the actin-resistant variant resulted in a significant decrease (p < 0.02) of the airway secretion contact angle and cough transport. A significantly higher (p < 0.01) increase in contact angle and cough transport of airway secretions was observed at 10 microg/ml with the actin-resistant variant as compared with the wild-type rhDNase. The present study had demonstrated that the actin-resistant rhDNase variant (A114R) has an enhanced capacity to improve the physical properties and cough transport of airway secretions from patients with cystic fibrosis.
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Dates et versions

inserm-00152115 , version 1 (06-06-2007)

Identifiants

  • HAL Id : inserm-00152115 , version 1
  • PUBMED : 11316652

Citer

Jm Zahm, C. Debordeaux, C. Maurer, D. Hubert, D. Dusser, et al.. Improved activity of an actin-resistant DNase I variant on the cystic fibrosis airway secretions.. American Journal of Respiratory and Critical Care Medicine, 2001, 163 (5), pp.1153-7. ⟨inserm-00152115⟩

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