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Absence of Dp71 in mdx3cv mouse spermatozoa alters flagellar morphology and the distribution of ion channels and nNOS.

Abstract : In muscle, the absence of dystrophin alters the dystrophin-associated protein complex (DAPC), which is involved in the clustering and anchoring of signaling proteins and ion and water channels. Here we show that mice spermatozoa express only dystrophin Dp71 and utrophin Up71. The purpose of this study was to explore the effect of the absence of Dp71 on the morphology and membrane distribution of members of the DAPC, ion channels and signaling proteins of spermatozoa obtained from dystrophic mutant mdx3cv mice. Our work indicates that although the absence of Dp71 results in a dramatic decrease in beta-dystroglycan, it induces membrane redistribution and an increase in the total level of alpha-syntrophin, voltage-dependent Na+ (micro1) and K+ (Kv1.1) channels and neural nitric oxide synthase (nNOS). The short utrophin (Up71) was upregulated and redistributed in the spermatozoa of mdx3cv mice. A significant increase in abnormal flagella morphology was observed in the absence of Dp71, which was partially corrected when the plasma membrane was eliminated by detergent treatment. Our observations point to a new phenotype associated with the absence of Dp71. Abnormal flagellar structure and altered distribution of ion channels and signaling proteins may be responsible for the fertility problems of mdx3cv mice.
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https://www.hal.inserm.fr/inserm-00150331
Contributor : Dominique Mornet <>
Submitted on : Thursday, November 19, 2009 - 8:38:21 AM
Last modification on : Friday, March 27, 2020 - 9:20:05 AM
Long-term archiving on: : Thursday, April 8, 2010 - 6:19:57 PM

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Enrique O Hernández-González, Dominique Mornet, Alvaro Rendon, Dalila Martínez-Rojas. Absence of Dp71 in mdx3cv mouse spermatozoa alters flagellar morphology and the distribution of ion channels and nNOS.. Journal of Cell Science, Company of Biologists, 2005, 118 (Pt 1), pp.137-45. ⟨10.1242/jcs.01584⟩. ⟨inserm-00150331⟩

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