Skip to Main content Skip to Navigation
Journal articles

E-Cadherin mediates MMP down-regulation in highly invasive bronchial tumor cells.

Abstract : The disorganization of E-cadherin/catenin complexes and the overexpression of matrix metalloproteinases (MMPs) are frequently involved in the capacity of epithelial cells to acquire an invasive phenotype. The functional link between E-cadherin and MMPs was studied by transfecting invasive bronchial BZR tumor cells with human E-cadherin cDNA. Using different in vitro (cell dispersion, modified Boyden chamber) and in vivo assays (human airway epithelial xenograft), we showed that E-cadherin-positive clones displayed a decrease of invasive abilities. As shown by immunoprecipitation, the re-expressed E-cadherin was able to sequestrate one part of free cytoplasmic beta-catenin in BZR cells. The decrease of beta-catenin transcriptional activity in E-cadherin-transfected clones was demonstrated using the TOP-FLASH reporter construct. Finally, we observed a decrease of MMP-1, MMP-3, MMP-9, and MT1-MMP, both at the mRNA and at the protein levels, in E-cadherin-positive clones whereas no changes in MMP-2, TIMP-1, or TIMP-2 were observed when compared with control clones. Moreover, zymography analysis revealed a loss of MMP-2 activation ability in E-cadherin-positive clones treated with the concanavalin A lectin. These data demonstrate a direct role of E-cadherin/catenin complex organization in the regulation of MMPs and suggest an implication of this regulation in the expression of an invasive phenotype by bronchial tumor cells.
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00149014
Contributor : Philippe Birembaut <>
Submitted on : Thursday, May 24, 2007 - 9:57:28 AM
Last modification on : Wednesday, August 30, 2017 - 1:13:29 AM

Identifiers

  • HAL Id : inserm-00149014, version 1
  • PUBMED : 12875984

Collections

Citation

Béatrice Nawrocki-Raby, Christine Gilles, Myriam Polette, Corinne Martinella-Catusse, Noël Bonnet, et al.. E-Cadherin mediates MMP down-regulation in highly invasive bronchial tumor cells.. American Journal of Pathology, American Society for Investigative Pathology, 2003, 163 (2), pp.653-61. ⟨inserm-00149014⟩

Share

Metrics

Record views

175