Skip to Main content Skip to Navigation
Journal articles

Linking PCNA-dependent replication and ATR by human Claspin.

Abstract : Recent studies in Xenopus have identified a new checkpoint protein called Claspin that is believed to transduce the checkpoint DNA damage signals to Chk1 kinase. Here we show that the human Claspin homolog is a chromatin bound protein either in the absence or in the presence of damaged DNA, independent of its association with ATR. Furthermore, we show that human Claspin is found in complex with PCNA, an essential component of the DNA replication machinery, and is released upon DNA replication arrest. Interfering with PCNA function by overexpression of p21 mutant, impaired in its interaction with Cdks but not with PCNA, leads to ATR-dependent Chk1 activation. These findings suggest that the dissociation of Claspin-PCNA could be part of the signal leading to Chk1 activation.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00147047
Contributor : Yves Le Ster <>
Submitted on : Tuesday, May 15, 2007 - 5:31:02 PM
Last modification on : Thursday, September 10, 2020 - 3:00:10 PM
Long-term archiving on: : Wednesday, April 7, 2010 - 12:57:16 AM

Identifiers

Citation

Jean-Marc Brondello, Bernard Ducommun, Anne Fernandez, Ned Lamb. Linking PCNA-dependent replication and ATR by human Claspin.. Biochemical and Biophysical Research Communications, Elsevier, 2007, 354 (4), pp.1028-33. ⟨10.1016/j.bbrc.2007.01.091⟩. ⟨inserm-00147047⟩

Share

Metrics

Record views

294

Files downloads

1220