Skip to Main content Skip to Navigation
Journal articles

Protein kinase C-zeta mediates retinal degeneration in response to TNF.

Abstract : Tumor necrosis factor-alpha (TNF) has been implicated in retinal ganglion cells (RGC) degeneration in glaucoma. Atypical protein kinase C (PKC) zeta is involved in cell protection against various stresses. The aim of this study was to investigate the potential proapoptotic effects of intravitreal injections of TNF with or without PKCzeta specific inhibitor on the rat retina. TNF was injected in the vitreous of rat eyes alone or in combination with specific PKCzeta inhibitor. PKCzeta and NF-kappaB were studied by immunohistochemistry and western-blotting analysis on retina, and apoptosis quantified by the TUNEL assay. While low basal PKCzeta was observed in the control eyes, TNF induced intense expression of PKCzeta mostly in bipolar cells processes. PKCzeta staining became nuclear when TNF was coinjected with PKCzeta inhibitor. TNF alone did not induce apoptosis in the retina. Coinjection of the PKCzeta-specific inhibitor and TNF, however, induced apoptosis in the inner nuclear and ganglion cell layers. The PKCzeta-specific inhibitor unmasks retinal cells to TNF cytotoxicity showing a link between the proapoptotic effects of TNF and the antiapoptotic PKCzeta signaling pathway.
Complete list of metadata

Cited literature [42 references]  Display  Hide  Download
Contributor : Patricia Crisanti Lassiaz <>
Submitted on : Monday, May 14, 2007 - 6:50:37 PM
Last modification on : Thursday, December 10, 2020 - 12:35:35 PM
Long-term archiving on: : Friday, November 25, 2016 - 3:39:51 PM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document




Hong Liang, Christophe Baudouin, Francine Behar-Cohen, Patricia Crisanti, Boubaker Omri. Protein kinase C-zeta mediates retinal degeneration in response to TNF.. Journal of Neuroimmunology, Elsevier, 2007, 183 (1-2), pp.104-10. ⟨10.1016/j.jneuroim.2006.11.028⟩. ⟨inserm-00146368⟩



Record views