Skip to Main content Skip to Navigation
Journal articles

Intramolecular processing of prothermolysin.

Abstract : Thermolysin, an extracellular zinc endopeptidase from Bacillus thermoproteolyticus, is synthesized as a pre-proenzyme and the prosequence has been shown to assist the refolding of the denatured enzyme in vitro and to inhibit enzyme activity (O'Donohue, M. J., and Beaumont, A. (1996) J. Biol. Chem. 271, 26477-26481). To determine whether prosequence cleavage from the mature enzyme is autocatalytic and if so, whether it is an intermolecular or intramolecular process, N-terminal histidine-tagged prothermolysin was expressed in Escherichia coli. Although partial processing to mature enzyme occurred, most of the proenzyme was recovered intact from inclusion bodies. This was then solubilized in guanidinium hydrochloride, immobilized on a cobalt-containing resin, and after dialysis against renaturation buffer, was quantitatively transformed to mature enzyme. However, when a mutation was introduced into the mature sequence to inactivate thermolysin, the proenzyme was not processed either in vivo or in vitro. In addition, mutated prothermolysin was not processed by exogenous thermolysin under a variety of experimental conditions. The results demonstrate that thermolysin maturation can proceed via an autocatalytic intramolecular pathway.
Document type :
Journal articles
Complete list of metadatas

Cited literature [26 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00145189
Contributor : Cynthia Marie-Claire <>
Submitted on : Wednesday, May 9, 2007 - 10:19:06 AM
Last modification on : Saturday, September 19, 2020 - 4:26:56 AM
Long-term archiving on: : Friday, November 25, 2016 - 3:27:57 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

  • HAL Id : inserm-00145189, version 1
  • PUBMED : 9488701

Collections

Citation

Cynthia Marie-Claire, Bernard Roques, Ann Beaumont. Intramolecular processing of prothermolysin.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 1998, 273 (10), pp.5697-701. ⟨inserm-00145189⟩

Share

Metrics

Record views

219