Skip to Main content Skip to Navigation
Journal articles

Kinetic analysis of tubulin assembly in the presence of the microtubule-associated protein TOGp.

Abstract : The microtubule-associated protein TOGp, which belongs to a widely distributed protein family from yeasts to humans, is highly expressed in human tumors and brain tissue. From purified components we have determined the effect of TOGp on thermally induced tubulin association in vitro in the presence of 1 mm GTP and 3.4 m glycerol. Physicochemical parameters describing the mechanism of tubulin polymerization were deduced from the kinetic curves by application of the classical theoretical models of tubulin assembly. We have calculated from the polymerization time curves a range of parameters characteristic of nucleation, elongation, or steady state phase. In addition, the tubulin subunits turnover at microtubule ends was deduced from tubulin GTPase activity. For comparison, parallel experiments were conducted with colchicine and taxol, two drugs active on microtubules and with tau, a structural microtubule-associated protein from brain tissue. TOGp, which decreases the nucleus size and the tenth time of the reaction (the time required to produce 10% of the final amount of polymer), shortens the nucleation phase of microtubule assembly. In addition, TOGp favors microtubule formation by increasing the apparent first order rate constant of elongation. Moreover, TOGp increases the total amount of polymer by decreasing the tubulin critical concentration and by inhibiting depolymerization during the steady state of the reaction.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00144754
Contributor : Yves Le Ster <>
Submitted on : Monday, May 7, 2007 - 5:31:45 PM
Last modification on : Wednesday, January 15, 2020 - 1:05:07 AM
Long-term archiving on: : Wednesday, April 7, 2010 - 12:11:50 AM

Identifiers

Collections

Citation

Claude Bonfils, Nicole Bec, Benjamin Lacroix, Marie-Cécile Harricane, Christian Larroque. Kinetic analysis of tubulin assembly in the presence of the microtubule-associated protein TOGp.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2007, 282 (8), pp.5570-81. ⟨10.1074/jbc.M605641200⟩. ⟨inserm-00144754⟩

Share

Metrics

Record views

532

Files downloads

735