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Two-Photon Water-Soluble Dyes and Their Amine-Reactive Derivatives for Two-Photon Bio-Imaging Applications

Abstract : Two series of water-soluble blue fluorescent two-photon excited chromophores for bio-imaging based on bis-stilbenic structure were synthesized. Wadsworth-Emmons reaction was used to build one dimensional D-π-aromatic core-π-D structures. The water solubility is induced by three oligo-ethylene glycol moieties in positions 3, 4, and 5 of the peripheral phenyl ring. Their two-photon absorption (TPA) cross-section in the range of 150 GM at 700 nm have been measured by the way of their two-photon excited fluorescence (TPEF) properties. It is 10 to 100 times higher than commercial dyes commonly used in bio-imaging. This makes these new TPA chromophores good candidates for in-vivo two-photon excited (TPE) microscopy.
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https://www.hal.inserm.fr/inserm-00144707
Contributor : Alain Duperray <>
Submitted on : Friday, May 4, 2007 - 2:56:42 PM
Last modification on : Thursday, April 23, 2020 - 2:26:22 PM

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  • HAL Id : inserm-00144707, version 1

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Ali Hayek, Frédéric Bolze, Jean-François Nicoud, Alain Duperray, Alexei Grichine, et al.. Two-Photon Water-Soluble Dyes and Their Amine-Reactive Derivatives for Two-Photon Bio-Imaging Applications. Nonlinear optics, quantum optics , Old City Pub., 2006, 35 (1-3), pp.155-164. ⟨inserm-00144707⟩

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