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Journal articles
A polypyrrole protein microarray for antibody-antigen interaction studies using a label-free detection process.
Abstract : Protein microarray is a promising technology that should combine rapidity and easy use with high throughput and versatility. This article describes a method in which an electrocopolymerization process is employed to graft biological molecules on to a chip so that surface plasmon resonance imaging may be used to detect molecular interactions. Copolymerization of pyrrole-modified protein and pyrrole is an efficient grafting process which immobilizes molecules at defined positions on a gold surface. Surface plasmon resonance imaging is an optical technique that allows real-time simultaneous detection of molecular interactions on a large number of spots without labeling. This method was successfully used to analyze antibody-antigen interactions. This illustrates its high specificity and good sensitivity and demonstrates its suitability for biological studies.
Cited literature [25 references]
https://www.hal.inserm.fr/inserm-00144353
Contributor : Patrice Marche <>
Submitted on : Thursday, May 3, 2007 - 7:21:00 AM
Last modification on : Wednesday, September 16, 2020 - 5:39:40 PM
Long-term archiving on: : Friday, November 25, 2016 - 3:45:50 PM
Contributor : Patrice Marche <>
Submitted on : Thursday, May 3, 2007 - 7:21:00 AM
Last modification on : Wednesday, September 16, 2020 - 5:39:40 PM
Long-term archiving on: : Friday, November 25, 2016 - 3:45:50 PM