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A polypyrrole protein microarray for antibody-antigen interaction studies using a label-free detection process.

Abstract : Protein microarray is a promising technology that should combine rapidity and easy use with high throughput and versatility. This article describes a method in which an electrocopolymerization process is employed to graft biological molecules on to a chip so that surface plasmon resonance imaging may be used to detect molecular interactions. Copolymerization of pyrrole-modified protein and pyrrole is an efficient grafting process which immobilizes molecules at defined positions on a gold surface. Surface plasmon resonance imaging is an optical technique that allows real-time simultaneous detection of molecular interactions on a large number of spots without labeling. This method was successfully used to analyze antibody-antigen interactions. This illustrates its high specificity and good sensitivity and demonstrates its suitability for biological studies.
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https://www.hal.inserm.fr/inserm-00144353
Contributor : Patrice Marche <>
Submitted on : Thursday, May 3, 2007 - 7:21:00 AM
Last modification on : Wednesday, September 16, 2020 - 5:39:40 PM
Long-term archiving on: : Friday, November 25, 2016 - 3:45:50 PM

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Ludivine Grosjean, Boutheina Cherif, Emilie Mercey, André Roget, Yves Levy, et al.. A polypyrrole protein microarray for antibody-antigen interaction studies using a label-free detection process.. Analytical Biochemistry, Elsevier Masson, 2005, 347 (2), pp.193-200. ⟨10.1016/j.ab.2005.09.033⟩. ⟨inserm-00144353⟩

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