Skip to Main content Skip to Navigation
Journal articles

Comparative transductions of breast cancer cells by three DNA viruses.

Abstract : Defining the ideal vectors to transduce breast cancer using viruses is currently under intense pre-clinical evaluation. Our study constitutes the first direct comparison of the infection efficiencies of a human serotype 5 (Ad5), a canine serotype 2 (CAV-2) adenovirus, and a human serotype 2 adeno-associated virus (AAV-2) in breast cancer cells. We observed an excellent infection efficiency for Ad5 vector, whereas both CAV-2 and AAV-2 vectors lead to low infection of these cells. Real-time PCR, flow cytometry, and antibody blocking studies suggest that Ad5 and CAV-2 infection ability is not strictly dependent on coxsackie adenovirus receptor (CAR) or alpha(v) integrin levels. In conclusion, our data suggest that human adenoviruses are excellent transducers of breast cancer cells, though it may be difficult to predict the extent of infection solely on CAR or alpha(v) integrin levels.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00143970
Contributor : Gwendal Lazennec <>
Submitted on : Monday, April 30, 2007 - 3:12:00 PM
Last modification on : Thursday, November 12, 2020 - 2:30:07 PM
Long-term archiving on: : Wednesday, April 7, 2010 - 3:29:41 AM

Identifiers

Citation

Annick Lucas, Eric Kremer, Silvio Hemmi, José Luis, Françoise Vignon, et al.. Comparative transductions of breast cancer cells by three DNA viruses.. Biochemical and Biophysical Research Communications, Elsevier, 2003, 309 (4), pp.1011-6. ⟨10.1016/j.bbrc.2003.08.101⟩. ⟨inserm-00143970⟩

Share

Metrics

Record views

325

Files downloads

2458