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Inhibition of PrPSc formation by lentiviral gene transfer of PrP containing dominant negative mutations.

Abstract : Currently, there is no treatment to cure transmissible spongiform encephalopathies. By taking advantage of the 'prion-resistant' polymorphisms Q171R and E219K that naturally exist in sheep and humans, respectively, we have evaluated a therapeutic approach of lentiviral gene transfer. Here, we show that VSV-G (vesicular stomatitis virus G glycoprotein) pseudotyped FIV-(feline immunodeficiency virus) derived vectors carrying the mouse Prnp gene in which these mutations have been inserted, are able to inhibit prion replication in chronically prion-infected cells. Because lentiviral tools are able to transduce post-mitotic cells such as neurons or cells of the lymphoreticular system, this result might help the development of gene- or cell-therapy approaches to prion disease.
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Submitted on : Thursday, April 26, 2007 - 11:25:56 AM
Last modification on : Friday, May 15, 2020 - 12:22:03 PM
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Carole Crozet, Yea-Lih Lin, Clément Mettling, Chantal Mourton-Gilles, Pierre Corbeau, et al.. Inhibition of PrPSc formation by lentiviral gene transfer of PrP containing dominant negative mutations.. Journal of Cell Science, Company of Biologists, 2004, 117 (Pt 23), pp.5591-7. ⟨10.1242/jcs.01484⟩. ⟨inserm-00143203⟩

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