Skip to Main content Skip to Navigation
Journal articles

Two types of store-operated Ca2+ channels with different activation modes and molecular origin in LNCaP human prostate cancer epithelial cells.

Abstract : The one or more coupling mechanisms of store-operated channels (SOCs) to endoplasmic reticulum (ER) Ca2+ store depletion as well as the molecular identity of SOCs per se still remain a mystery. Here, we demonstrate the co-existence of two populations of molecular distinct endogenous SOCs in LNCaP prostate cancer epithelial cells, which are preferentially activated by either active inositol 1,4,5-trisphosphate (IP3)-mediated or passive thapsigargin-facilitated store depletion and have different ER store content sensitivity. The first population, called SOC(CC) (for "conformational coupling"), is characterized by preferential IP3 receptor-dependent mode of activation, as judged from sensitivity to cytoskeleton modifications, and dominant contribution of transient receptor potential (TRP) TRPC1 within it. The second one, called SOC(CIF) (for "calcium influx factor"), depends on Ca(2+)-independent phospholipase A2 for activation with probable CIF involvement and is mostly represented by TRPC4. The previously identified SOC constituent in LNCaP cells, TRPV6, seems to play equal role in both SOC populations. These results provide new insight into the nature of SOCs and their representation in the single cell type as well as permit reconciliation of current SOC activation hypotheses.
Document type :
Journal articles
Complete list of metadatas

Cited literature [61 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00139796
Contributor : Matthieu Flourakis <>
Submitted on : Tuesday, April 3, 2007 - 3:30:15 PM
Last modification on : Thursday, February 21, 2019 - 10:30:02 AM
Long-term archiving on: : Friday, November 25, 2016 - 4:11:18 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Fabien Vanden Abeele, Loic Lemonnier, Stéphanie Thébault, Gilbert Lepage, Jan Parys, et al.. Two types of store-operated Ca2+ channels with different activation modes and molecular origin in LNCaP human prostate cancer epithelial cells.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2004, 279 (29), pp.30326-37. ⟨10.1074/jbc.M400106200⟩. ⟨inserm-00139796⟩

Share

Metrics

Record views

232