 |
Journal articles
A major role for Mcl-1 antiapoptotic protein in the IL-6-induced survival of human myeloma cells.
Abstract : Interleukin-6 (IL-6) is a major survival factor for malignant plasma cells involved in multiple myeloma. Using an RNase protection assay, we looked for gene expression of 10 anti- and proapoptotic Bcl-2-family proteins in 12 IL-6-dependent human myeloma cell lines (HMCL). A high Mcl-1 gene expression was found in all HMCLs and the other genes were variably expressed. Out of the 10 Bcl-2-family members, only the Mcl-1 gene was regulated by IL-6. Upon starvation of IL-6, Mcl-1 gene expression decreased in association with myeloma cell apoptosis and was upregulated after adding IL-6 again in association with myeloma cell survival. A constitutive Mcl-1 expression was induced with an Mcl-1-GFP retrovirus in two IL-6-dependent HMCLs. The Mcl-1 HMCLs have a marked reduced apoptosis upon IL-6 starvation compared to HMCLs transduced with control GFP retrovirus and may grow without adding IL-6. These data emphasize the major role of Mcl-1 antiapoptotic protein in the IL-6-induced survival of human myeloma cells.
Cited literature [26 references]
https://www.hal.inserm.fr/inserm-00130855
Contributor : Monique Frei <>
Submitted on : Wednesday, February 14, 2007 - 4:21:48 PM
Last modification on : Friday, October 19, 2018 - 7:30:08 PM
Long-term archiving on: : Tuesday, April 6, 2010 - 11:55:22 PM
Contributor : Monique Frei <>
Submitted on : Wednesday, February 14, 2007 - 4:21:48 PM
Last modification on : Friday, October 19, 2018 - 7:30:08 PM
Long-term archiving on: : Tuesday, April 6, 2010 - 11:55:22 PM