A silanized hydroxypropyl methylcellulose hydrogel for the three-dimensional culture of chondrocytes.

Abstract : Articular cartilage has limited intrinsic repair capacity. In order to promote cartilage repair, the amplification and transfer of autologous chondrocytes using three-dimensional scaffolds have been proposed. We have developed an injectable and self-setting hydrogel consisting of hydroxypropyl methylcellulose grafted with silanol groups (Si-HPMC). The aim of the present work is to assess both the in vitro cytocompatibility of this hydrogel and its ability to maintain a chondrocyte-specific phenotype. Primary chondrocytes isolated from rabbit articular cartilage (RAC) and two human chondrocytic cell lines (SW1353 and C28/I2) were cultured into the hydrogel. Methyl tetrazolium salt (MTS) assay and cell counting indicated that Si-HPMC hydrogel did not affect respectively chondrocyte viability and proliferation. Fluorescent microscopic observations of RAC and C28/I2 chondrocytes double-labeled with cell tracker green and ethidium homodimer-1 revealed that chondrocytes proliferated within Si-HPMC. Phenotypic analysis (RT-PCR and Alcian blue staining) indicates that chondrocytes, when three-dimensionnally cultured within Si-HPMC, expressed transcripts encoding type II collagen and aggrecan and produced sulfated glycosaminoglycans. These results show that Si-HPMC allows the growth of differentiated chondrocytes. Si-HPMC therefore appears as a potential scaffold for three-dimensional amplification and transfer of chondrocytes in cartilage tissue engineering.
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Article dans une revue
Biomaterials, Elsevier, 2005, 26 (33), pp.6643-51. 〈10.1016/j.biomaterials.2005.04.057〉
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Contributeur : Georges Carle <>
Soumis le : lundi 30 octobre 2006 - 11:03:21
Dernière modification le : vendredi 31 août 2018 - 13:59:32

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Claire Vinatier, David Magne, Pierre Weiss, Christophe Trojani, Nathalie Rochet, et al.. A silanized hydroxypropyl methylcellulose hydrogel for the three-dimensional culture of chondrocytes.. Biomaterials, Elsevier, 2005, 26 (33), pp.6643-51. 〈10.1016/j.biomaterials.2005.04.057〉. 〈inserm-00110465〉

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