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A silanized hydroxypropyl methylcellulose hydrogel for the three-dimensional culture of chondrocytes.

Abstract : Articular cartilage has limited intrinsic repair capacity. In order to promote cartilage repair, the amplification and transfer of autologous chondrocytes using three-dimensional scaffolds have been proposed. We have developed an injectable and self-setting hydrogel consisting of hydroxypropyl methylcellulose grafted with silanol groups (Si-HPMC). The aim of the present work is to assess both the in vitro cytocompatibility of this hydrogel and its ability to maintain a chondrocyte-specific phenotype. Primary chondrocytes isolated from rabbit articular cartilage (RAC) and two human chondrocytic cell lines (SW1353 and C28/I2) were cultured into the hydrogel. Methyl tetrazolium salt (MTS) assay and cell counting indicated that Si-HPMC hydrogel did not affect respectively chondrocyte viability and proliferation. Fluorescent microscopic observations of RAC and C28/I2 chondrocytes double-labeled with cell tracker green and ethidium homodimer-1 revealed that chondrocytes proliferated within Si-HPMC. Phenotypic analysis (RT-PCR and Alcian blue staining) indicates that chondrocytes, when three-dimensionnally cultured within Si-HPMC, expressed transcripts encoding type II collagen and aggrecan and produced sulfated glycosaminoglycans. These results show that Si-HPMC allows the growth of differentiated chondrocytes. Si-HPMC therefore appears as a potential scaffold for three-dimensional amplification and transfer of chondrocytes in cartilage tissue engineering.
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Contributor : Georges Carle <>
Submitted on : Monday, October 30, 2006 - 11:03:21 AM
Last modification on : Monday, October 12, 2020 - 10:27:35 AM



Claire Vinatier, David Magne, Pierre Weiss, Christophe Trojani, Nathalie Rochet, et al.. A silanized hydroxypropyl methylcellulose hydrogel for the three-dimensional culture of chondrocytes.. Biomaterials, Elsevier, 2005, 26 (33), pp.6643-51. ⟨10.1016/j.biomaterials.2005.04.057⟩. ⟨inserm-00110465⟩



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