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A simple and highly sensitive radioenzymatic assay for lysophosphatidic acid quantification.

Abstract : The objective of the present work was to develop a simple and sensitive radioenzymatic assay to quantify lysophosphatidic acid (LPA). For that, a recombinant rat LPA acid acyltransferase (LPAAT) produced in Escherichia coli was used. In the presence of [(14)C]oleoyl-CoA, LPAAT selectively catalyzes the transformation of LPA and alkyl-LPA into [(14)C]phosphatidic acid. Acylation of LPA was complete and linear from 0 to 200 pmol with a minimal detection of 0.2 pmol. This method was used to quantify LPA in butanol-extracted lipids from bovine sera, as well as from human and mouse plasma.This radioenzymatic assay represents a new, simple, and highly sensitive method to quantify LPA in various biological fluids.
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https://www.hal.inserm.fr/inserm-00110161
Contributor : Jean Sébastien Saulnier-Blache <>
Submitted on : Thursday, November 2, 2006 - 9:03:36 AM
Last modification on : Friday, January 10, 2020 - 9:09:06 PM
Long-term archiving on: : Tuesday, April 6, 2010 - 9:06:34 PM

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  • HAL Id : inserm-00110161, version 1
  • PUBMED : 11108727

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Jean Sébastien Saulnier-Blache, Alexia Girard, Marie-Françoise Simon, Max Lafontan, Philippe Valet. A simple and highly sensitive radioenzymatic assay for lysophosphatidic acid quantification.. J Lipid Res, 2000, 41 (12), pp.1947-51. ⟨inserm-00110161⟩

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