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Targeting of single stranded oligonucleotides through metal-induced cyclization of short complementary strands: Targeting of single stranded oligonucleotides

Abstract : A new strategy to cyclize a short synthetic oligonucleotide on a DNA or a RNA target strand is described. This one relies on a metal-mediated cyclization of short synthetic oligonucleotides conjugated with two chelating 2,2':6',2”-terpyridine moieties at their 3' and 5' ends. Cyclization following metal addition (Zn2+, Fe2+) was demonstrated using UV monitored thermal denaturation experiments, mass spectrometry analysis and gel shift assays. NMR experiments were used to analyse the association of complementary strands after metal-mediated cyclization. We have thus demonstrated that an efficient circularization of synthetic oligonucleotides is easily achieved through this strategy. The hybridization on a complementary strand was more efficient with a RNA target strand and a 2'-O-methyl circularized oligomer.
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https://www.hal.inserm.fr/inserm-00107329
Contributor : Serge Moreau <>
Submitted on : Tuesday, May 11, 2010 - 12:17:32 PM
Last modification on : Wednesday, November 29, 2017 - 2:53:58 PM
Long-term archiving on: : Thursday, September 16, 2010 - 12:02:54 PM

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Fabrice Freville, Tristan Richard, Katell Bathany, Serge Moreau. Targeting of single stranded oligonucleotides through metal-induced cyclization of short complementary strands: Targeting of single stranded oligonucleotides. Helvetica Chimica Acta, Wiley, 2006, 89 (12), pp.2958-2974. ⟨10.1002/hlca.200690265⟩. ⟨inserm-00107329⟩

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