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Journal Articles Biomedecine & Pharmacotherapy Year : 2006

An LNA-based loss-of-function assay for micro-RNAs.

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Abstract

MicroRNAs (miRNAs) have recently emerged as being essential for development and for the control of cell proliferation/differentiation in various organisms. However, little is known about miRNA function and mode of action at the cellular level. We have designed a miRNA loss-of-function assay, based on chemically modified locked nucleic acids (LNA) antisense oligonucleotides and usable in tissue culture cells. We show that LNA/DNA mixed oligonucleotides form highly stable duplexes with miRNAs in vitro. Ex vivo, the target miRNA becomes undetectable in cells transfected with the antisense oligonucleotide. The effect is dose-dependent, long-lasting, and specific. Moreover, using a reporter assay, we show that antisense LNA/DNA oligonucleotides inhibit short non-coding RNAs at the functional level. Thus LNA/DNA mixmers represent powerful tools for functional analysis of miRNAs.

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inserm-00091652 , version 1 (06-09-2006)

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Irina Naguibneva, Maya Ameyar-Zazoua, Nora Nonne, Anna Polesskaya, Slimane Ait-Si-Ali, et al.. An LNA-based loss-of-function assay for micro-RNAs.. Biomedecine & Pharmacotherapy, 2006, 60 (9), pp.633-8. ⟨10.1016/j.biopha.2006.07.078⟩. ⟨inserm-00091652⟩

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