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Cytosolic type II estrogen binding site in rat uterus: specific photolabeling with estrone.

Abstract : A low affinity (Kd = 30 nM), large capacity (Bmax = 2.6 pmol/g tissue) estrogen binding site was photolabeled from estradiol-stimulated rat uterus cytosol. To maximize levels of this binding site and reduce those of the type I binding site, ovariectomized rats were injected with high doses of estradiol (10 micrograms per day) for four days with the last injection two hours before sacrifice. This treatment depleted type I estrogen receptors from the cytosol (by 90%) and raised levels of type II sites in the nucleus without affecting cytosolic type II levels. The type II estradiol binding sites were distinguished from the type I sites on the basis of their dissociation kinetics, pH-sensitivity and their behavior towards potassium chloride, somatostatin, sodium thiocyanate, sulfhydryl reagents and ammonium sulfate precipitation. These type II binding sites could be covalently photolabeled with tritiated estrone. A molecular weight of 43 kDa was found on SDS PAGE.
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https://www.hal.inserm.fr/inserm-00090797
Contributor : Marc Poirot <>
Submitted on : Monday, September 4, 2006 - 1:37:58 AM
Last modification on : Monday, September 4, 2006 - 3:39:03 PM

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  • HAL Id : inserm-00090797, version 1
  • PUBMED : 1583622

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Marc Poirot, Catherine Chailleux, Francis Bayard, Jean-Charles Faye. Cytosolic type II estrogen binding site in rat uterus: specific photolabeling with estrone.. J Recept Res, 1992, 12, pp.217-31. ⟨inserm-00090797⟩

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