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Insulin and estrogen receptor ligand influence the FGF-2 activities in MCF-7 breast cancer cells.

Abstract : From the MCF-7 cell line we have developed, a human mammary cancer cell subline with the same karyotype as the mother strain and named MCF-7(SF), able to grow in serum-free chemically defined medium. This cell subline was firstly used to analyze the effect of basic fibroblast growth factor (FGF-2) in estrogen-receptor-positive human breast cancer cells. FGF-2 like estradiol is able to increase cell proliferation and pS2 expression but was also found to inhibit progesterone receptor (PR) expression. The anti-estrogen tamoxifen partly counteracts the effects of FGF-2 and to discriminate between its two main mediators (estrogen receptor vs. anti-estrogen binding site, AEBS) we compare the efficacies of pure anti-estrogen (ICI 182,780) and AEBS ligand (PBPE). It appears that pure anti-estrogen counteracts cell growth and pS2 effects of FGF-2 since AEBS ligand inhibits the cell growth but has no activity on pS2 expression. Secondly, adding insulin (10(-6)M) in the culture medium induces a strong increase in cell proliferation, which then elicits an inhibitory effect of FGF-2 and addition of anti-estrogens, are less efficient to further decrease growth, since the effects of FGF-2 and anti-estrogens on pS2 expression are conserved.
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Contributor : Marc Poirot Connect in order to contact the contributor
Submitted on : Monday, September 4, 2006 - 1:12:37 AM
Last modification on : Wednesday, June 1, 2022 - 4:57:13 AM


  • HAL Id : inserm-00090793, version 1
  • PUBMED : 12566092


Martine Garnier, Claire Giamarchi, Isabelle Delrieu, Marie-Christine Rio, Patrick Chinestra, et al.. Insulin and estrogen receptor ligand influence the FGF-2 activities in MCF-7 breast cancer cells.. Biochemical Pharmacology, Elsevier, 2003, 65, pp.629-36. ⟨inserm-00090793⟩



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