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Role of the T cell receptor alpha chain in the development and phenotype of naturally arising CD4+CD25+ T cells.

Abstract : The T cell receptor alpha chain repertoire and the possible influence of the alpha chain on the development and phenotype of naturally arising mouse CD4+CD25+ T cells have not been extensively analysed. We used all available Valpha-specific monoclonal antibodies and a sensitive multiplex genomic DNA PCR assay to study the Valpha repertoire of CD4+CD25+ T cells in normal mice. To address whether CD4+CD25+ T cells express two TCR alpha chains, we have carried out four-colour flow cytometry using combinations of the available anti-Valpha reagents in mice where one allele of the TCRA locus had been inactivated. Results indicate that the Valpha repertoire of CD4+CD25+ T cells is as diverse as their CD25- partners. In addition, CD4+CD25+ T cells develop normally in Tcralpha+/- mice and we show for the first time that despite expressing only one TCRalpha chain, they retain their characteristic CD4(low), CD3(low), TCRbeta(low), CD5(high), CD45RB(low) and cytoplasmic CD152(high) phenotype.
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https://www.hal.inserm.fr/inserm-00089361
Contributor : Patrice Marche <>
Submitted on : Thursday, August 17, 2006 - 5:56:06 PM
Last modification on : Friday, November 6, 2020 - 3:52:07 AM

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Nabil Bosco, Hsiu-Cheng Hung, Nicolas Pasqual, Evelyne Jouvin-Marche, Patrice Marche, et al.. Role of the T cell receptor alpha chain in the development and phenotype of naturally arising CD4+CD25+ T cells.. Molecular Immunology, Elsevier, 2006, 43 (3), pp.246-54. ⟨10.1016/j.molimm.2005.02.002⟩. ⟨inserm-00089361⟩

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