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Journal articles
Electronic sorting and recovery of single live cells from microlitre sized samples.
Alexandra Fuchs
1
Aldo Romani
2
Delphine Freida
1
Gianni Medoro
3
Mélanie Abonnenc
3
Luigi Altomare
2
Isabelle Chartier
4
Dorra Guergour
5
Christian Villiers
5
Patrice Marche
5
Marco Tartagni
2
Roberto Guerrieri
2
Francois Chatelain
1
Nicolo Manaresi
3
Mélanie Abonnenc 3
Author
Luigi Altomare 2
Author
Isabelle Chartier 4
Author
Dorra Guergour 5
Author
Christian L. Villiers 5
Author
Patrice Marche 5
Author IdHAL : patrice-n-marche ResearcherId : http://www.researcherid.com/rid/K-5060-2013 ORCID : https://orcid.org/0000-0002-8930-9340
Marco Tartagni 2
Author
Roberto Guerrieri 2
Author
Francois Chatelain 1
Author
Nicolo Manaresi 3
Author
1
BIOPUCES - Laboratoire Biopuces (France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DSV/IRTSV/BIOPUCES (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
2
ARCES - Advanced Research Center on Electronic Systems for Information and Communication Technologies E. De Castro [Bologna] (Via Toffano,2 - 40125 Bologna - Italy)
- UNIBO - Alma Mater Studiorum Università di Bologna [Bologna] (Via Zamboni, 33 - 40126 Bologna - Italy)
4
CEA-LETI - Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (MINATEC 17, rue des Martyrs, 38054, Grenoble Cedex 9 - France)
- DRT (CEA) - Direction de Recherche Technologique (CEA) : DRT (France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DRT (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
5
Contrôle moléculaire de la réponse immune specifique (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U548 (101, rue de Tolbiac, 75013 Paris - France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DSV/IRTSV (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- UJF - Université Joseph Fourier - Grenoble 1 (BP 53 - 38041 Grenoble Cedex 9 - France)
Abstract : Sorting and recovering specific live cells from samples containing less than a few thousand cells have become major hurdles in rare cell exploration such as stem cell research, cell therapy and cell based diagnostics. We describe here a new technology based on a microelectronic chip integrating an array of over 100,000 independent electrodes and sensors which allow individual and parallel single cell manipulation of up to 10,000 cells while maintaining viability and proliferation capabilities. Manipulation is carried out using dynamic dielectrophoretic traps controlled by an electronic interface. We also demonstrate the capabilities of the chip by sorting and recovering individual live fluorescent cells from an unlabeled population.
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Journal articles
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https://www.hal.inserm.fr/inserm-00089360
Contributor : Patrice Marche <>
Submitted on : Thursday, August 17, 2006 - 5:36:40 PM
Last modification on : Friday, November 6, 2020 - 3:52:07 AM
Contributor : Patrice Marche <>
Submitted on : Thursday, August 17, 2006 - 5:36:40 PM
Last modification on : Friday, November 6, 2020 - 3:52:07 AM
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Identifiers
- HAL Id : inserm-00089360, version 1
- DOI : 10.1039/b505884h
- PUBMED : 16372078
Citation
Alexandra Fuchs, Aldo Romani, Delphine Freida, Gianni Medoro, Mélanie Abonnenc, et al.. Electronic sorting and recovery of single live cells from microlitre sized samples.. Lab on a Chip, Royal Society of Chemistry, 2006, 6 (1), pp.121-6. ⟨10.1039/b505884h⟩. ⟨inserm-00089360⟩
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