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Clinically related protein-peptide interactions monitored in real time on novel peptide chips by surface plasmon resonance imaging.

Abstract : BACKGROUND: Developing rapid, high-throughput assays for detecting and characterizing protein-protein interactions is a great challenge in the postgenomic era. We have developed a new method that allows parallel analysis of multiple analytes in biological fluids and is suitable for biological and medical studies. METHODS: This technology for studying peptide-antibody interactions is based on polypyrrole-peptide chips and surface plasmon resonance imaging (SPRi). We generated a chip bearing a large panel of peptide probes by successive electro-directed copolymerizations of pyrrole-peptide conjugates on a gold surface. RESULTS: We provide evidence that (a) the signal produced by antibody binding is highly specific; (b) the detected signal specifically reflects the antibody concentration of the tested solution in a dose-dependent manner; (c) this technique is appropriate for analyzing complex media such as undiluted sera, a novelty with respect to previous techniques; and (d) correlation between classic ELISA results and the SPRi signal is good (P = 0.008). We also validated this system in a medical model by detecting anti-hepatitis C antibodies in patient-derived sera. CONCLUSION: Because of its characteristics (easy preparation of the peptide chip; high-throughput, label-free, real-time detection; high specificity; and low background), this technology is suitable for screening biological samples and for large-scale studies.
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https://www.hal.inserm.fr/inserm-00089356
Contributor : Patrice Marche <>
Submitted on : Thursday, August 17, 2006 - 5:38:15 PM
Last modification on : Friday, November 6, 2020 - 3:50:01 AM

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Boutheima Cherif, André Roget, Christian Villiers, Roberto Calemczuk, Vincent Leroy, et al.. Clinically related protein-peptide interactions monitored in real time on novel peptide chips by surface plasmon resonance imaging.. Clinical Chemistry, American Association for Clinical Chemistry, 2006, 52 (2), pp.255-62. ⟨10.1373/clinchem.2005.058727⟩. ⟨inserm-00089356⟩

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