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Detection and localization of a Ca2+-ATPase activity in Toxoplasma gondii.

Abstract : Toxoplasma gondii, the agent causing toxoplasmosis, is an obligate intracellular protozoan parasite. A calcium signal appears to be essential for intracellular transduction during the active process of host cell invasion. We have looked for a Ca2+-transport ATPase in tachyzoites and found Ca2+-ATPase activity (11-22 nmol Pi liberated/mg protein/min) in the tachyzoite membrane fraction. This ATP-dependent activity was stimulated by Ca2+ and Mg2+ ions and by calmodulin, and was inhibited by pump inhibitors (sodium orthovanadate or thapsigargin). We used cytochemistry and X-ray microanalysis of cerium phosphate precipitates and immunolabelling to find the Ca2+, Mg2+-ATPase. It was located mainly in the membrane complex, the conoid, nucleus, secretory organelles (rhoptries, dense granules) and in vesicles with a high calcium concentration. Thus, Toxoplasma gondii possesses Ca2+-pump ATPase (Ca2+, Mg2+-ATPase) as do eukaryotic cells.
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https://www.hal.inserm.fr/inserm-00078996
Contributor : Dominique Laurent-Maquin <>
Submitted on : Thursday, June 8, 2006 - 2:48:50 PM
Last modification on : Tuesday, October 31, 2017 - 2:58:02 PM

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  • HAL Id : inserm-00078996, version 1
  • PUBMED : 11345503

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André Bouchot, Jean-David Jaillet, Annie Bonhomme, Nathalie Pezzella-d' Alessandro, Patrice Laquerriere, et al.. Detection and localization of a Ca2+-ATPase activity in Toxoplasma gondii.. Cell Struct Funct, 2001, 26, pp.49-60. ⟨inserm-00078996⟩

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