Detection and localization of a Ca2+-ATPase activity in Toxoplasma gondii. - Archive ouverte HAL Access content directly
Journal Articles Cell Struct Funct Year : 2001

Detection and localization of a Ca2+-ATPase activity in Toxoplasma gondii.

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Abstract

Toxoplasma gondii, the agent causing toxoplasmosis, is an obligate intracellular protozoan parasite. A calcium signal appears to be essential for intracellular transduction during the active process of host cell invasion. We have looked for a Ca2+-transport ATPase in tachyzoites and found Ca2+-ATPase activity (11-22 nmol Pi liberated/mg protein/min) in the tachyzoite membrane fraction. This ATP-dependent activity was stimulated by Ca2+ and Mg2+ ions and by calmodulin, and was inhibited by pump inhibitors (sodium orthovanadate or thapsigargin). We used cytochemistry and X-ray microanalysis of cerium phosphate precipitates and immunolabelling to find the Ca2+, Mg2+-ATPase. It was located mainly in the membrane complex, the conoid, nucleus, secretory organelles (rhoptries, dense granules) and in vesicles with a high calcium concentration. Thus, Toxoplasma gondii possesses Ca2+-pump ATPase (Ca2+, Mg2+-ATPase) as do eukaryotic cells.
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Dates and versions

inserm-00078996 , version 1 (08-06-2006)

Identifiers

  • HAL Id : inserm-00078996 , version 1
  • PUBMED : 11345503

Cite

André Bouchot, Jean-David Jaillet, Annie Bonhomme, Nathalie Pezzella-d' Alessandro, Patrice Laquerriere, et al.. Detection and localization of a Ca2+-ATPase activity in Toxoplasma gondii.. Cell Struct Funct, 2001, 26, pp.49-60. ⟨inserm-00078996⟩

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