In vitro release of vascular endothelial growth factor from gadolinium-doped biodegradable microspheres. - Archive ouverte HAL Access content directly
Journal Articles Magnetic Resonance in Medicine Year : 2004

In vitro release of vascular endothelial growth factor from gadolinium-doped biodegradable microspheres.

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Abstract

A drug delivery vehicle was constructed that could be visualized noninvasively with MRI. The biodegradable polymer poly(DL-lactic-co-glycolic acid) (PLGA) was used to fabricate microspheres containing vascular endothelial growth factor (VEGF) and the MRI contrast agent gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA). The microspheres were characterized in terms of size, drug and contrast agent encapsulation, and degradation rate. The PLGA microspheres had a mean diameter of 48 +/- 18 microm. The gadolinium loading was 17 +/- 3 microg/mg polymer and the VEGF loading was 163 +/- 22 ng/mg polymer. Electron microscopy revealed that the Gd was dispersed throughout the microspheres and it was confirmed that the Gd loading was sufficient to visualize the microspheres under MRI. VEGF and Gd-DTPA were released from the microspheres in vitro over a period of approximately 6 weeks in three phases: a burst, followed by a slow steady-state, then a rapid steady-state. Biodegradable Gd-doped microspheres can be effectively used to deliver drugs in a sustained manner, while being monitored noninvasively with MRI.

Dates and versions

inserm-00078812 , version 1 (07-06-2006)

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Anthony Z. Faranesh, Monet T. Nastley, Cristina Perez de La Cruz, Michael F. Haller, Patrice Laquerriere, et al.. In vitro release of vascular endothelial growth factor from gadolinium-doped biodegradable microspheres.. Magnetic Resonance in Medicine, 2004, 51, pp.1265-71. ⟨10.1002/mrm.20092⟩. ⟨inserm-00078812⟩

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