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Behavior of human osteoblast-like cells in contact with electrodeposited calcium phosphate coatings.

Abstract : Calcium-deficient hydroxyapatite (Ca-def-HAP) thin films were elaborated on Ti6Al4V substrates by electrodeposition. The coatings exhibit two different morphologies and crystallinities. Human osteoblast-like cells (MG-63) were cultured on the surfaces of these materials; the cell content and viability were evaluated up to 28 days. The scanning electron microscopy and biological investigations showed cells with a normal morphology, good proliferation, and viability from 7 to 21 days. But after 28 days, the number of live cells decreases in both cases; however, this decrease is less important in the case of calcium phosphate (CaP) coating surface when compared with the control (cell culture plastic). The cells cultured on Ca-def-HAP coating exhibit more cellular extensions and extracellular matrix. RT-PCR for type I collagen, alkaline phosphatase, and osteocalcin studies were also carried out, and was found that the CaP enhances gene expression of ALP and OC and thus the differentiation of osteoblast-like cells. Moreover, this study shows that the difference in the morphology of CaP coatings has no effect on the biocompatibility. (c) 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006.
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Contributor : Dominique Laurent-Maquin Connect in order to contact the contributor
Submitted on : Wednesday, May 31, 2006 - 4:18:15 PM
Last modification on : Thursday, October 14, 2021 - 1:10:03 PM

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Doriane Richard, Nicolas Dumelié, Hicham Benhayoune, Sylvie Bouthors, Christine Guillaume, et al.. Behavior of human osteoblast-like cells in contact with electrodeposited calcium phosphate coatings.. Journal of Biomedical Materials Research Part B: Applied Biomaterials, Wiley, 2006, 79(1), pp.108-15. ⟨10.1002/jbm.b.30519⟩. ⟨inserm-00077680⟩



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