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MMP-2, MMP-9 and their inhibitors TIMP-2 and TIMP-1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles.

Abstract : After calcium-phosphates biomaterials based implantation like hydroxyapatite (HA) coating, particles are released in the periprosthetic tissues. Wear-debris induced fibrous membranes contain macrophage subsets that can produce metalloproteinases (MMPs), which are considered to be key enzymes in extra-cellular matrix turnover. Tissue inhibitors of metalloproteinases (TIMPs) are important regulator of MMPs activity. Interleukin-1 mainly produced by monocytes can also regulate MMPs production. In the present work, we have evaluated the effect of HA particles characteristics (size, shape and sintering temperature) on the MMP-2, -9 and their respective inhibitors TIMP-2, -1 production. Our results demonstrate that sintering temperature (that modify crystal size and surface area) have little effect on MMPs and TIMPs production. Non-phagocytable particles induced more MMP-9, although phagocytable particles induced more IL-1β release. The shape of the particles was the most important factor since needle-shaped particles induced the most significant up-regulated expression of MMPs and IL-1β.
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https://www.hal.inserm.fr/inserm-00068633
Contributor : Dominique Laurent-Maquin <>
Submitted on : Tuesday, June 6, 2006 - 11:40:18 AM
Last modification on : Thursday, January 11, 2018 - 6:16:29 AM
Long-term archiving on: : Saturday, April 3, 2010 - 11:22:21 PM

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  • HAL Id : inserm-00068633, version 1
  • PUBMED : 14751736

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Patrice Laquerriere, Alexia Grandjean-Laquerriere, Salima Addadi-Rebbah, Edouard Jallot, Dominique Laurent-Maquin, et al.. MMP-2, MMP-9 and their inhibitors TIMP-2 and TIMP-1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles.. Biomaterials, Elsevier, 2004, 25 (13), pp.2515-24. ⟨inserm-00068633⟩

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