 |
Journal articles
MMP-2, MMP-9 and their inhibitors TIMP-2 and TIMP-1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles.
Abstract : After calcium-phosphates biomaterials based implantation like hydroxyapatite (HA) coating, particles are released in the periprosthetic tissues. Wear-debris induced fibrous membranes contain macrophage subsets that can produce metalloproteinases (MMPs), which are considered to be key enzymes in extra-cellular matrix turnover. Tissue inhibitors of metalloproteinases (TIMPs) are important regulator of MMPs activity. Interleukin-1 mainly produced by monocytes can also regulate MMPs production. In the present work, we have evaluated the effect of HA particles characteristics (size, shape and sintering temperature) on the MMP-2, -9 and their respective inhibitors TIMP-2, -1 production. Our results demonstrate that sintering temperature (that modify crystal size and surface area) have little effect on MMPs and TIMPs production. Non-phagocytable particles induced more MMP-9, although phagocytable particles induced more IL-1β release. The shape of the particles was the most important factor since needle-shaped particles induced the most significant up-regulated expression of MMPs and IL-1β.
Cited literature [31 references]
https://www.hal.inserm.fr/inserm-00068633
Contributor : Dominique Laurent-Maquin <>
Submitted on : Tuesday, June 6, 2006 - 11:40:18 AM
Last modification on : Tuesday, April 20, 2021 - 12:00:12 PM
Long-term archiving on: : Saturday, April 3, 2010 - 11:22:21 PM
Contributor : Dominique Laurent-Maquin <>
Submitted on : Tuesday, June 6, 2006 - 11:40:18 AM
Last modification on : Tuesday, April 20, 2021 - 12:00:12 PM
Long-term archiving on: : Saturday, April 3, 2010 - 11:22:21 PM